The XML list above will generate a simple menu that appears as:Ĭurrently, the control doesn't have an XSD file, but perhaps in future revisions, I'll get around to adding one for added functionality. Under each of the TopLevelMenu elements are MenuItem elements which also have N-level support. The application can support N number of TopLevelMenu elements. It uses a root element to encompass the TopLevelMenu elements. There's really nothing too extreme about the XML structure. The main purpose of this control is to generate an applications menu via a MenuStrip control, at runtime, based on values provided in an XML configuration file that can be located in a file system folder or embedded as a resource directly in to your application. I'm sure there are other interesting things to note as well. For a more detailed explanation, check out Jim Hollenhorst's CodeProject article: " Upgrading from MainMenu and ToolBar to MenuStrip and ToolStrip." Things to NoteĪside from showing how to enhance the MenuStrip control to use an XML file as its data source, this control also shows some pretty basic techniques for using Reflection, loading files from embedded resources, parsing an XML file, and organizing an XML file. There are some noticeable differences in the two. One main thing here is that this control uses the new, relatively speaking. Of course, I'm sure there are many more pros and cons that you can come up with, but these were just a few that I came up with off the top of my head.
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![]() Midnight comes into the classroom to help explain how their names will be tied to their professional career. ![]() ![]() Hero names will be temporary, but they’ll have to be serious with it. ![]() The reckless use of his quirk must be the cause of that.īut regardless if they’ve received any offers, students are still expected to join internships to hone their experience and productivity as future heroes. Uraraka feels excited to be one of the top selections. Todoroki’s offers come first place with Bakugo in second. Students should expect calls from pro-hero agencies by now, which may continue on until their graduation period.Īs expected, Bakugo and Todoroki gain the most offers, with the others distributed in lower numbers. Aizawa comes in with his wounds fully healed and announces the need for everyone to pick their hero code names. The students of 1A share stories about their newfound popularity after the televised sports festival. He tells Midoriya that there’s nothing to be worried about his brother. That morning, he finds Iida running to school with a raincoat on. Regardless of when it’s released, fans are eagerly awaiting the return of their favorite heroes.The school festival break ends with Midoriya fully healing from his wounds and now it’s time to return to class. While the release date has not yet been officially announced, fans can expect the new season to be released sometime in 2024, based on the release schedule of past seasons. With new characters and casts being introduced, it’s sure to be an exciting season. The announcement of My Hero Academia Season 7 has left fans of the show excited for what’s to come. If you’re a fan who can’t wait for the anime and want to dive into the manga, you can start reading from chapter 329 onwards. As for the anime adaptation, Season 6 has covered up to chapter 328 of the manga, meaning there are currently 55+ chapters worth of material available for fans to explore. The My Hero Academia Manga is an ongoing series and currently has over 383 chapters. If you’re wondering where to start with the My Hero Academia Season 7 Manga, you might be curious about how many chapters the anime has covered and how many chapters are available currently. Where to Begin with My Hero Academia Season 7 Manga? Whatever the case may be, fans are eagerly waiting for the release of MHA Season 7. My Hero Academia Season 7 Teaser VisualĪlternatively, they may decide to produce a single-core season with 12 or 13 episodes, which would allow them to release the new season earlier. With not enough source material for a 2-cour season, if the production team decides to wait for the manga to finish first, then fans might have to wait a little longer for the new season. However, there’s also a possibility that the release schedule may be different this time because the anime has almost caught up to the manga. The gap between each season has been approximately one and a half years, and if they follow this trend, the upcoming season will likely be released around the same time. While the release date of My Hero Academia Season 7 has not been officially announced, fans can expect it to be released in Spring 2024, based on the release schedule of past seasons. Romi Park expressed her excitement about being a part of the show and promised to put her heart and soul into the role. Romi Park, who has voiced many famous characters such as “Fullmetal Alchemist” Edward Elric and “Attack on Titan” Hanji Zoe, will voice Star and Stripe in the upcoming season. Fans were given a glimpse of the character in the final episode of season 6. One of the most exciting new characters is Star and Stripe, the No. ![]() Or, serve this as a side to your Italian dinner (hint: our sheet-pan panzanella or creamy chicken marsala make for dream pairings). If you're serving this up as a crowd-pleasing party app, pair it with our toasted ravioli and baked feta bites to complete your spread. ![]() If you’re worried about your tomato mixture sliding off the toasted bread, consider a schmear before you top them (think pureed white beans, cream cheese, or whipped ricotta).īruschetta was made for lazy summer days, so it goes perfectly with other easy, fresh snacks like our melon-prosciutto skewers or classic Caprese salad. We toasted our slices until beautifully golden and just crisp enough to create the perfect base for our tomatoes before rubbing them with halved garlic cloves to really amp up the fresh, bright flavor. Ciabatta is a popular option, but we went with a large, sliced baguette in this recipe. You really want a crisp, sturdy base that will hold up your tomato mixture without falling apart. ![]() If you're preparing bruschetta with out-of-season tomatoes, this salting trick drastically improves their quality! Let sit for 5 minutes, and the liquid will gather at the bottom of your bowl. After they have been diced, add them to a large strainer or colander over a bowl, and toss with some salt. When you use too juicy tomatoes, you will no doubt get a powerful (and delicious) tomato result, but your toast might end up soggy. Move over martinis This ceviche recipe offers a quick and easy cocktail when looking for a light lunch or appetizer. The ultimate goal is to keep your toast like toast should be: dry. Add spaghetti and cook according to package directions until. Juicier tomatoes work, but drier varieties like Roma are really what you’re looking for. 4 green onions, thinly sliced 1 In a large pot of salted boiling water, add 2 tablespoons Old Bay. The key to making the best bruschetta? It's all in the tomatoes.īecause tomatoes are the focus of this simple recipe, it’s worth it to use ripe, fresh ones. Read on for all of our top tips on acing this classically refreshing side: It’s also the perfect make-ahead snack: The longer the tomatoes sit in the balsamic-garlic-olive oil mixture, the better the flavors meld and develop. While you can make it all year round, the best time to enjoy this appetizer is summer, when fresh tomatoes are at their peak. Looking for a special date night? Start your steak dinner off right by serving this classy app first.Easy to throw together and made up of simple, classic ingredients, bruschetta was made for chill warm weather snacking. Paired with some hard-boiled eggs, we like to. Pair with our bacon wrapped dates and stuffed mushrooms if you're planning an extra-fancy party, or with classic Southern potato salad and fried okra for a casual BBQ vibe. Shrimp, Avocado, and Egg Chopped Salad Seafood lovers, rejoice Shrimp is high in protein and low in calories and carbs, and cooks up quickly. With ketchup, chili sauce, Worcestershire sauce and celery salt, this condiment packs a flavorful punch perfect for dunking your party app in. Peanut Sauce Soba with Crispy Tofu Stuffed Turkey Breast Air Fryer Fish and Chips Scallion Pancake Hasselback Potatoes Lemony Garlic and Herb Pork Tenderloin Roasted Asparagus With Creamy Feta. We gave this party standby a well-needed upgrade with our zesty, homemade sauce. Although our recipe comes with an extra-flavorful sauce, we didn't skimp on the seasoning for our shrimp either. Technically, the shrimp is seasoned twice here: once when the shrimp is boiled, and a second time when it hits the sauce right before you bite into it. If you've ever wondered: is shrimp cocktail served raw? The answer is no-the shrimp is boiled until pink and cooked through, then chilled before serving. Never made shrimp cocktail at home before? Follow our top tips on making this tried-and-true appetizer. Prepare a grill for medium-high heat heat 5 minutes (or heat a grill pan over medium-high heat). While you might be tempted to buy store-bought, trust us-making it homemade makes all the difference. To give this classic dish an upgrade, we transformed the classic cocktail sauce into a zesty, ultra-flavorful condiment you'll want to pair with all your seafood dinners. Step 1 If using wooden skewers, soak in water 30 minutes. Ready in under an hour, this shrimp appetizer is so easy to prepare, making it a perfect last-minute appetizer for any party you want to add some fancy flare to. It has all the simplicity of a casserole but with the flavors of a shrimp scampi so succulent, it would make Bubba Gump proud. ![]() While it may seem old-fashioned, it's remained a party app staple for a reason. Last week, our site's most popular recipe left us eagerly anticipating the arrival of sunshine and summer break. It's indisputable: Shrimp cocktail is a classic. To stress test the Framework Laptop 13, we put the system through 20 runs of Cinebench R23, simulating an extremely taxing workload. This was the fastest of the group, beating the MacBook Air (7:52) and the other devices. On Handbrake, the Framework transcoded a 4K video to 1080p in 5 minutes and 49 seconds. The SSD in the Framework Laptop 13 was blazing fast, copying 25GB files of 1,697.07 MBps, coming in just ahead of the Yoga 9i and far ahead of the MacBook Air. The Dell XPS 13 Plus (9320) with its Core i7-1280P has an impressive second-place multi-core showing at 10,621. The Lenovo Yoga 9i, with a Core i7-1360P, fell behind at 1,827/9,954. On Geekbench 5, the Framework Laptop 13 earned a single-core score of 1,958 (inching past the MacBook Air) and a multi-core score of 11,317, the highest of the group. This likely can keep the Intel version cheaper, or perhaps Framework just has a stock of DDR4 RAM it wants to get rid of. Intel's Raptor Lake supports DDR5, but Framework hasn't chosen to go that direction (though it will on its upcoming AMD option). I have to imagine that extra RAM was a boon. The "professional" version of the Framework Laptop 13, with an Intel Core i7-1370P, a whopping 32GB of RAM and a 1TB M.2 PCIe SSD did well on our benchmarks. Productivity Performance on the Framework Laptop 13 (Intel) For those using the mainboard as a computer without the battery (perhaps as a maker project), the company says these batteries will be available in the Framework Marketplace. In a blog post earlier this year, Framework wrote that shipping without the battery "reduces environmental impact." If you unplug the main battery, the system clock will reset, but Framework says it will catch up on the time or date when your OS reconnects to the internet. One interesting missing item is the CMOS battery, sometimes referred to as the RTC battery. It has the latest Intel CPU (though maybe we'd want to change it to try an AMD mainboard that’s coming later this year). Many of the repairs or upgrades aren't necessary on our unit yet. While this goes against the advice most laptop vendors offer, I admit I've done plenty of minor repairs without removing the battery connector. One QR code of interest specifically tells you to keep the battery connected for most repairs due to the "sensitive" connector it uses. You can scan parts with your phone as you repair them to go directly to maintenance manuals. Just about every part has some sort of QR code on it. “Delay any nonessential travel during storms,” Drabinski advised residents of the Santa Cruz Mountains. Meanwhile, Caltrans District 5 spokesperson Kevin Drabinski told the Sentinel that crews are focused on clearing out debris from culverts and other drainage infrastructure in the Santa Cruz Mountains, as blockages can lead to saturation, flooding and roadway slip outs. However, he expects to escalate that to “Level 1″ by Friday morning, which will fully staff in-person emergency operations so it can provide speedy response services. Reid said Wednesday that the county’s Emergency Operations Center was activated at “Level 2″ meaning that county staff is engaged in emergency planning and preparedness. Santa Cruz County Public Works Director Matt Machado told the Sentinel during the January storms that he hits his “nervous button” when the gauge reaches 28.5 feet, as that level of water puts significant pressure on the levee.Īfter observing seepage along the river’s earthen levee in early January, the county began emergency repairs in two separate sections to support the levee’s structure. “If you were impacted by flooding in January you should be prepared for evacuation warnings and orders and be prepared for potential flooding associated with this storm,” said Reid, adding that the San Lorenzo and Pajaro rivers along with Soquel, Aptos, Corralitos and Salsipuedes creeks are all being monitored closely.Īs of Wednesday afternoon, the Pajaro River at Chittenden is expected to enter its “action” stage early Saturday, topping out at 27.5 feet, according to the prediction center. Santa Cruz County Office of Response, Recovery and Resilience Director Dave Reid told the Sentinel that county officials are expecting the storm’s strength will measure up to other storms that struck the county in January and said the community should prepare for flood conditions in low-lying areas. Wind speeds will range from 20 to 30 mph with gusts as high as 50 mph, according to the forecast. The weather service has also issued a wind advisory beginning 1 p.m. “Especially since soils are still saturated, not only is flooding a big concern but so are down trees and power lines,” Dalton Behringer, meteorologist with the National Weather Service, told the Sentinel Wednesday. Coastal ranges and inland hills may get 3-6 inches, while urban regions could also see totals of 1.5-3 inches. Rainfall totals at high terrain areas in the Santa Cruz Mountains are estimated at 6-8 inches and as much as 10 inches at favored peaks. Thank you for listening to the weather report from LocalConditions.SANTA CRUZ - Another atmospheric river storm is barreling toward the Central Coast and, once again, Santa Cruz County finds itself directly within its flight path.Īccording to a forecast from the National Weather Service, rainfall is expected to arrive late Thursday morning with heavy downpours Thursday afternoon that will continue into Friday morning. Calm wind becoming south 5 to 7 mph in the afternoon. Saturday has an expected forecast of Partly sunny, then gradually becoming sunny, with a high near 72. Otherwise, mostly cloudy, with a low around 49. The forecast for Tonight is Patchy fog before 3am. ![]() The moon phase is a Waxing Crescent Moon. Sunrise is at 5:54 AM and Sunset is at 8:12 PM. ![]() Wind direction is East at 4 miles per hour, gusting at 7 mph. The humidity is 93.6 percent with a dew point of 51.8 degrees fahrenheit that is steady since the last report. The barometric pressure is 30.1 - measured by inch of mercury units - and is steady since its last observation. It is 54 degrees fahrenheit, or 12 degrees celsius and feels like 53 degrees fahrenheit. The current weather report for Santa Cruz County CA, as of 1:55 AM PDT, has a sky condition of Overcast with the visibility of 9.00 miles. ![]() ![]() ![]() Join us around the fireside in our music/event field with melodic tunes to rock your soul underneath the stars in the heartbeat of the New River Gorge National Park.įree Event Live Music with Alexander Nicole at the Freefolk Brewery Live Music at the Tap House! Join us for local, live music, delicious food and tantalising cocktails & craft beer! Fireside Jam with Dina Hornbaker at the Outpost NRG Live Music with Ken Kruger Southside Junction Tap House The race will feature a mass start from Cunard to Fayette Station, and participants will compete in classes of three or more of their most common paddlers and crafts. The Gorge Race is an intermediate/advanced class IV kayak and raft race in the New River Gorge. All scores will be added together with awards going to the highest scoring participants. At the end of the day the judges will meet collectively to review the video and score each competitor again. Judges will score participants at each location. At flows over 20,000 cfs we will move the event to a primary surf competition on the New River Dries Put-in Waves. The rolling rodeo will consist of a ramp competition, river feature surfing and downriver trick competition scheduled to take place at Cunard to Lower Railroad Rapid on the New River. As part of the adventure offered at the New River Gorge Festival it’s your chance to compete with local boaters and legendary paddlers in West Virginia. Each class ends in a meditation suitable for littles.īook NEW RIVER GORGE RACE & ROLLING RODEOĪre you the fastest paddler in the New River Gorge? Or maybe you love surfing every wave on the river and seeing how many tricks you can throw in each rapid? This May ACE Adventure Gear and ACE Adventure Resort present the New River Gorge Race & Rolling Rodeo Competition. It is about having fun while learning about mindfulness, meditation, and yoga. Children are introduced to yoga asanas (poses) in age-appropriate ways including through games and imagination. Join us for some fun, calming, and interactive kids yoga classes!Įach kids yoga class with Katie starts with ‘getting out of our heads and onto our mats’ and a breath/mindfulness exercise. Local producers will be set up with a variety of seasonal vegetables, microgreens, breads, jellies, baked goods, meats, eggs, honey, maple syrup, plants, flowers, soaps, candles arts and more. The Fayetteville Farmers Market is set up every Saturday from 8am -12:30pm off Wiseman Ave in the Fayette County Courthouse parking lot. Greg Riordan is BACK at Freefolk Friday May 12th 7-9pm! Live Music Charlie’s Pubĩpm at Charlie’s Pub Saturday, May 13 Fayette County Farmers MarketĨam-12:30pm W Wiseman Ave. Outdoor classes are weather permitting.īook Live Music with Greg Riordan at the Freefolk Brewery Information Friday, May 12 Jordan’s Creative Vinyasa Flow YogaĮnjoy yoga overlooking the spectacular views of the New River Gorge on the Adventures on the Gorge Pool deck. Not anymore! Meet friends new and old in the New River Gorge this spring and experience a weekend full of outdoor fun, adventure films, live music, vendors, camping, and more! Many rivers have their own events such as Gauley Fest, Cheat Fest, StoneyFest, and MooseFest, but somehow the New River got left out. Let’s celebrate one of Earth’s oldest rivers that we all love to paddle, hike, bike, and explore. The New River Gorge Festival is back May 12th- 14th at ACE Adventure Resort in West Virginia. May 12-14 Ace Adventure Resort NEW RIVER GORGE FESTIVAL ![]() They are similar in shape to freshwater discuss, but they have a long flowing dorsal fin that sets them apart. Their character is based on the Moorish Idol. Gill is the name of the grizzled veteran in the aquarium. Likeness rating: 7/10 Just like its on-screen representation, the Moorish Idol is a hardy fish. It doesn’t hurt that they are hardy and relatively easy to keep.Ĭool fact: The dorsal spine grows longer as the fish ages They have a beautiful, elegant swimming style which has helped Tangs become popular aquarium mainstays. It is from the Tang family (as is Bubbles) similarly, it feeds by grazing on rocks and feeding off tiny creatures such as Copepods. The Blue Tang is known as Paracanthurus hepatus in Latin. Dory’s real-life counterpart is the Blue Tang or sometimes the Regal Tang. She is wonderfully voiced by Ellen DeGeneres. ![]() Likeness rating: 9/10 Dory’s character really suits the actual regal tangĭory is the loveable and forgetful sidekick of Marlin. The reefs that they inhabit are typically at shallow depths.Ĭool fact: The spine of a Regal Tang is incredibly sharp and can be erected as a self-defense In the wild, these fish like to live in coral reefs. This species is popular among aquarium hobbyists, although, like all tangs, they can grow quite large, so they need a lot of space. This fish is based around the yellow tang. Bubbles get excited whenever the treasure chest in the tank pops open, and bubbles come out. Likeness rating: 7/10 The yellow tang does not seem to have an affinity to bubbles in reality, unlike Bubblesīubbles is the bright yellow fish that lives in the aquarium in the dentist’s office. Their sharp teeth need more than some seagrass or seaweed.Ĭool fact: When sleeping, Yellow Tangs sometimes attach themselves to rocks with their scalpels to prevent floating away However, unlike their animated counterparts, real-life sharks seem to have little intention of taking up a vegetarian lifestyle. Great Whites are known to frequent much of the southern coast of Australia (perhaps the reason why the filmmakers chose the accent they did for the character). Looking at the coloration of this character, you might assume that it is a Great White shark as they are known for their counter-shading (that is, having a light underbelly and a dark top, so they are camouflaged from both positions). Likeness rating: 8/10 The real-life Great White is a fierce predator, just like Bruce in Finding Nemo ![]() However, they might be a bit too big and adventurous to keep in your fish tank.Ĭool fact: It can sense a drop of blood in 3 miles It seems like Disney went all out to give the movie a quasi-realistic edge. As for the babies being carried around on their mother’s backs, believe it or not, that is a behavior exhibited by some species, although mainly on the land. These well-loved ocean creatures are some of the best-known animals on this list. Likeness rating: 9/10 The majestic sea turtle, for which Crush and Squirt are So which creatures were the original cast based on? Let’s take a look at some of the more popular “members of the cast”.Ĭool fact: Sea turtles don’t have a favorite food (though most eat jellyfish or seaweed) Many of these characters can be found in your home aquarium as well as in the wild. The fish found in the classic Disney movie Finding Nemo are not just from the imagination of the animators. ![]() The Level 2 upgrade of the Sand Boots requires five Hightail Lizards. The Level 2 upgrades of the Climber's Bandanna, Climbing Gear, and Climbing Boots each require five Hightail Lizards, along with five Electric Keese Wings. In particular, Hightails Lizards can be used to enhance the Climbing Set and the Sand Boots. This material can be used to enhance Link's Armor in the game. If Link cooks the Hightail Lizard along with food, it will result in Link making Dubious Food. This will temporarily boost Links movement speed. When cooked with a monster part that has no specific enhancement, such as a Bokoblin Horn, Link will create a Hasty Elixir. The Hightail Lizard will give Link a Speed enhancement. Do not combine two different ingredients that provide two different buffs because they. Drink the Fireproof Elixir to prevent Link from catching on fire. Two Fireproof Lizards and a Bokoblin Horn. You can increase the level of potency of the elixir by adding ingredients that provide the same buff. Four Hot-Footed Frogs and a Bokoblin Horn. Hightail Lizards can be cooked alongside monster parts to create Elixirs. The Hightail Lizard can be used as an elixir ingredient, since you can combine any Critter and Monster Part together into a Cooking Pot to make an elixir. You can also buy Hightail Lizards from Beedle at Lakeside Stable and South Akkala Stable. If Link moves too fast, the noise will scare the lizard away. Link can then very slowly walk over and grab the Hightail Lizard. This will cut a significant amount of the grass surrounding Link, and will often reveal a Hightail Lizard, along with Restless Crickets. Link can use a metallic weapon, such as a Soldier's Broadsword and charge up a spin attack. Most creatures drop recoverable materials and some are used for as ingredients for cooking, side. They often take a moment to register Link 's presence, but flee quickly once they do. ![]() 1 They can be found basking on Trees or Rocks, and they can also be startled out of hiding when Grass is cut. The Hightail Lizard will give Link a Speed enhancement. They can commonly found wherever there is tall grass. The Islander Hawk is a one of the creatures found in The Legend of Zelda: Breath of the Wild. Hightail Lizards are common Lizards that can be encountered in warm and temperate areas throughout Hyrule Kingdom. You can drink the Tough Elixir for a temporary boost to Link’s defenses.Hightail Lizards can commonly be obtained in many of the forests of the Great Plateau and the Necluda region. When you drink the Spicy Elixir, Link becomes more resistant to cold weather. Cook it with monster parts for a speed-boosting elixir. You can use this potion to increase Link’s stealth capabilities, making it harder to find him. It’s a bit slow to react at times, but if given a chance to escape, it will dart off quickly. PS: but you can also just shoot lizards with an arrow and pick them up afterwards. ![]() Your maximum hearts are also increased, but they go away after you take damage.įour Chuchu Jellies and a Bladed Rhino Beetleĭrinking this power increases Link’s attack power.įour Sunset Fireflies and a Bokoblin Fang Here is a full list of his different shop stocks. When you drink the Hasty Elixir, it gives Link increased movement speed while running, climbing, and swimming.ĭrink this Potion to fully restore Link’s health. Two Fireproof Lizards and a Bokoblin Hornĭrink the Fireproof Elixir to prevent Link from catching on fire.įour Hightail Lizards and a Bokoblin Horn Using the Electro Elixir Increases Link’s resistance to electricity.įour Restless Crickets and a Bokoblin Hornĭrinking the Energizing Elixir restores Link’s stamina.įour Hot-Footed Frogs and a Bokoblin Horn Elixir Nameįour Winterwing Butterflies and a Moblin Hornĭrinking the Chilly Elixir increases Link’s resistance to heat. Hightail Lizard Art from The Legend of Zelda: Breath of the Wild art artwork videogames gameart conceptart illustration legendofzelda zelda. We’ve also listed the various effects these Elixir give you when you use them. These are all of the Elixir recipes you can make in Breath of the Wild. The number of ingredients you use matters, and it’s important to remember this during the crafting process. You can do this at any cooking station you find while exploring the game, but it all comes down to the ingredients you’re using. These new platforms are able to generate large quantities of data in short periods of time, but until methods were developed for de novo assembly of large genomes from short read sequences, Sanger sequencing remained the standard method of creating a reference genome. Most high-throughput, next generation sequencing platforms produce shorter read lengths compared to Sanger sequencing. Scaffolding and next generation sequencing They concluded that the ALLPATHS-LG and SPAdes algorithms were superior to other assemblers in terms of the number of, maximum length of, and N50 length of contigs and scaffolds. investigated the utility of several different assembly software packages in combination with hybrid sequence data. A standalone program, GapFiller, is capable of closing a larger amount of gaps, using less memory than gap filling algorithms contained within assembly programs. Some software, like ABySS and SOAPdenovo, contain gap filling algorithms which, although they do not create any new scaffolds, serve to decrease the gap length between contigs of individual scaffolds. ALLMAPS is the first of such programs and is capable of combining data from genetic maps, created using SNPs or recombination data, with physical maps such as optical or synteny maps. In recent years, there has been an advent of new kinds of assemblers capable of integrating linkage data from multiple types of linkage maps. SSPACE is the most commonly cited assembly tool in biology publications, likely due to the fact that it is rated as a significantly more intuitive program to install and run than other assemblers. SSPACE also uses a greedy algorithm that begins building its first scaffold with the longest contig provided by the sequence data. The algorithm used by Bambus 2 removes repetitive contigs before orienting and ordering them into scaffolds. Bambus uses a greedy algorithm, defined as such because it joins together contigs with the most links first. Algorithms can be further classified as greedy, non greedy, conservative, or non conservative. Graph based applications have the capacity to order and orient over 10,000 markers, compared to the maximum 3000 markers capable of iterative marker applications. Īlgorithms used by assembly software are very diverse, and can be classified as based on iterative marker ordering, or graph based. This software also allowed for optional use of other linking data, such as contig order in a reference genome. ![]() Bambus was created in 2003 and was a rewrite of the original grouper software, but afforded researchers the ability to adjust scaffolding parameters. After the Human Genome Project and Celera proved that it was possible to create a large draft genome, several other similar programs were created. Until 2001, this was the only scaffolding software. The success of this strategy prompted the creation of the software, Grouper, which was included in genome assemblers. That project generated a total of 140 contigs, which were oriented and linked using paired end reads. The sequencing of the Haemophilus influenzae genome marked the advent of scaffolding. This can be done using either optical mapping or mate-pair sequencing. The next step is to then bridge the gaps between these contigs to create a scaffold. When creating a draft genome, individual reads of DNA are second assembled into contigs, which, by the nature of their assembly, have gaps between them. The sequences that are linked are typically contiguous sequences corresponding to read overlaps. ![]() Link together a non-contiguous series of genomic sequences into a scaffold, consisting of sequences separated by gaps of known length. ![]() ![]() Scaffolding is a technique used in bioinformatics. ![]() ![]() These pawns are essentially wild animals and can only be recruited through taming or rescuing. ![]() Slaver factions will also sell pawns at their own settlements if you are willing to travel. These will immediately join your colony on purchase and you can check the stats of each before you decide to buy, giving you more control over who you recruit. Slave caravans often have pawns to sell if you can afford to buy. They can also be enemies that you can capture. When an escape pod crashes onto your map tile, you will often get the chance to rescue a pawn from critical condition, and sometimes these pawns will choose to stay and join your colony. Both usually involve some sort of combat far from the safety of your colony and you can end up losing more pawns than you gain, so it's worth being cautious. These events require travel on the world map and are usually a risky way of acquiring a new pawn. In exchange for fighting off their pursuers, they will join your colony. This event will ask you to help a pawn fleeing from a hostile faction. A random pawn will join the colony and you have no say in it. This event is most likely if you are playing with under 4 colonists but can trigger up to the limit of 13. For this reason, if you're looking to build a giant colony, Randy is your best storyteller. It also becomes more difficult to recruit prisoners past this point. All storytellers will aim to give you between 4 and 13 colonists, where less will trigger events more often and then more will reduce them.Īfter you reach 18 colonists, Cassandra & Phoebe will both increase event difficulty in an attempt to bring your numbers down, while Randy will allow you to get up to 50. The storyteller you choose will then change the odds of events based on your population during the game. Depending on the start conditions you choose, you can have anywhere between 1 and 5 starting colonists with the standard start being 3. |